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Our laboratory is highly specialized in the preparation of soft animal tissue for electron microscopy (EM). We preserve clear morphological details even in difficult conditions such as infection, wound healing and others:


Infected endometrium. Mucus containing particulate matter covers the apices of many epithelial cells.



Wound repair in infected endometrium. Many red and white blood cells are seen inside the epithelial gap.





 
  Our techniques permit us to perform diverse high
quality serial examinations in the same sample:

-First scanning EM and secondly light microscopy

-First light microscopy and secondly scanning EM

-First scanning EM and secondly transmission EM






1. Light microscopy image of a piece of endometrium
previously examined by scanning EM.

2. Scanning EM image of a piece of endometrium
previously examined by light microscopy (recovered
from a paraffin block).

3. Transmission EM image of a piece of endometrium
previously examined by scanning EM.

  
       
     
         
 

SECTIONING We have developed a technique of sectioning tissue pieces that allows visualizing in SEM structures lying underneath the surface, such as epithelial basement membrane, stromal cells, vessels and other compartments, or even inside the cells. In most cases, a section runs through the epithelial cells exposing intracellular structures:


Endometrial gland surrounded by its basement membrane and stroma, exposed by the sectioning technique. This endometrium was infected and the lumen filled with mucus containing particulate matter.


Detail of the above figure. Epithelial cells are cut exposing intracellular structures and organelles. The basement membrane is on the left, and the apical membrane on the right, towards the lumen.


Rarely, a section exposes the lateral epithelial cell membranes intact. A basement membrane sheath lies at bottom.


Endometrial stroma exposed by sectioning. Some narrow corridors are seen containing red and white blood cells.


When two "sister" sectioned pieces are viewed stereoscopically side by side, it becomes obvious that their surfaces are perfectly complimentary and unspoiled from the cut.


Two "sister" pieces of endometrium obtained by sectioning. The cut runs through an endometrial gland.



Detail of the above figure, displaying the sectioned epithelium. Except for some slight displacement of the basement membrane, the newly exposed surfaces show absolute complementarity.

 
       
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